drosophila activity monitoring dam system  (Trikinetics Inc)

Journal: bioRxiv

Article Title: Modeling Post-traumatic memory deficits: Repeated head injury effects on Novelty detection in Drosophila

doi: 10.64898/2026.04.30.721976

Figure Lengend Snippet: Glia proliferation following repeated head trauma. ( A ) Immunohistochemistry of expression pattern of gal4 line used to label astrocyte-like glia in the Drosophila brain. ( B ) Flies showed no increase in astrocyte-like glia cell number one day after repeated head concussions. Conversely, flies showed a significant increase in astrocyte-like glia cell number five days after repeated head concussions with moderate and severe intensities. Finally, flies showed a significant increase in astrocyte-like glia cell number ten days after repeated head concussions with mild, moderate and severe intensities. One-way Anova, Kluskal-Wallis test followed by Dunn’s multiple comparison, *p<0.01, **p<0.0062, ****p<0.0001. ( C ) Representative images of glia labeled with multicolor flip-out approach.

Article Snippet: Drosophila sleep and activity were measured using the Drosophila activity monitoring system (Trikinetics) as described previously ( ; PJ et al., 2000).

Techniques: Immunohistochemistry, Expressing, Comparison, Labeling

Journal: bioRxiv

Article Title: Modeling Post-traumatic memory deficits: Repeated head injury effects on Novelty detection in Drosophila

doi: 10.64898/2026.04.30.721976

Figure Lengend Snippet: Repeated head trauma impairs neuronal plasticity in a well-defined olfactory circuit. ( A ) Left, diagram of in vivo functional imaging. Middle, experimental protocol. Flies expressing GCaMP6f in MBON-α3 (MB082-gal4) were trained by repeated presentation of 12, 1 s pulses of 1-octanol with a 12 s inter-stimulus interval (ISI), followed by 2 pulses of 4-methyl-cyclohexanol. Right, Immunohistochemistry of expression pattern of MB082-gal4 line used to label MBON-α3 in the Drosophila brain and live imaging view of ROI registered during olfactory recordings. ( B ) Functional imaging of normalized calcium traces of MBON-α3 responses upon repeated odor presentations (gray bars) in a naïve animal (red traces) and an animal following moderate repeated head trauma one day after concussions (magenta traces). Shaded area represents SEM. ( C ) Average calcium responses measured in ROI after 12 repeated presentations of OCT and 2 of MCH. Integrated ΔF/Fo signal was normalized for first response. Recordings were obtained in flies one, five- and ten-days post repeated head trauma with different head compressions, sham, mild, moderate and severe. An age-matched naïve control was also used. The error bars represent ± SEM in all figures. Bar graphs show the average of first three responses compared to the last three. As expected, naïve animals showed a significantly decreased responses when first three are compared to last three responses, indicating neuronal plasticity (habituation) after repeated odor presentations from day one to day 10. Interestingly, sham, mild moderate and severe treatments showed impaired neuronal plasticity one day post head trauma. Nevertheless, Sham flies recovered completely five and 10 days after, whereas mild moderate and severe concussed flies remain maintain impairment plasticity 5 days after head concussion. Finally, all flies seem to recover neuronal plasticity 10 days after repeated head trauma as indicated by a significant decrease in odor responses in last three odor presentations compared to first three. Matched-pair T-test, *p≤0.0388, **p≤0.0045 .

Article Snippet: Drosophila sleep and activity were measured using the Drosophila activity monitoring system (Trikinetics) as described previously ( ; PJ et al., 2000).

Techniques: Olfactory, In Vivo, Functional Assay, Imaging, Expressing, Immunohistochemistry, Control